PDBe 1req

X-ray diffraction
2Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Methylmalonyl-CoA mutase large subunit Chains: A, C
Molecule details ›
Chains: A, C
Length: 727 amino acids
Theoretical weight: 80.14 KDa
Source organism: Propionibacterium freudenreichii subsp. shermanii
Expression system: K38 PGP1-2
UniProt:
  • Canonical: P11653 (Residues: 2-728; Coverage: 100%)
Gene name: mutB
Sequence domains:
Structure domains:
Methylmalonyl-CoA mutase small subunit Chains: B, D
Molecule details ›
Chains: B, D
Length: 637 amino acids
Theoretical weight: 69.43 KDa
Source organism: Propionibacterium freudenreichii subsp. shermanii
Expression system: Escherichia coli
UniProt:
  • Canonical: P11652 (Residues: 2-638; Coverage: 100%)
Gene name: mutA
Sequence domains: Methylmalonyl-CoA mutase
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID2
Spacegroup: P21
Unit cell:
a: 119.8Å b: 161.3Å c: 88.4Å
α: 90° β: 105.1° γ: 90°
R-values:
R R work R free
0.22 0.22 0.275
Expression systems:
  • K38 PGP1-2
  • Escherichia coli