PDBe 1rc5

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF MG(II)-COMPLEX OF RNASE III ENDONUCLEASE DOMAIN FROM AQUIFEX AEOLICUS AT 2.30 ANGSTROM RESOLUTION

Released:
Source organism: Aquifex aeolicus

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphomonoester. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease 3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 154 amino acids
Theoretical weight: 18.16 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67082 (Residues: 1-147; Coverage: 67%)
Gene names: aq_946, rnc
Sequence domains: Ribonuclease III domain
Structure domains: Ribonuclease iii, N-terminal Endonuclease Domain; Chain A

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21
Unit cell:
a: 49.742Å b: 140.856Å c: 49.746Å
α: 90° β: 117.29° γ: 90°
R-values:
R R work R free
0.192 0.197 0.255
Expression system: Escherichia coli BL21(DE3)