PDBe 1ra6

X-ray diffraction
2Å resolution

Poliovirus Polymerase Full Length Apo Structure

Released:

Function and Biology Details

Reactions catalysed:
NTP + H(2)O = NDP + phosphate. 
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly. 
Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein. 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 461 amino acids
Theoretical weight: 52.99 KDa
Source organism: Human poliovirus 1 Mahoney
Expression system: Escherichia coli
UniProt:
  • Canonical: P03300 (Residues: 1749-2209; Coverage: 21%)
Sequence domains: RNA dependent RNA polymerase
Structure domains:

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P65
Unit cell:
a: 127.59Å b: 127.59Å c: 112.971Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.231 0.247
Expression system: Escherichia coli