PDBe 1r8g

X-ray diffraction
2.15Å resolution

Structure and function of YbdK

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative glutamate--cysteine ligase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 372 amino acids
Theoretical weight: 42.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77213 (Residues: 1-372; Coverage: 100%)
Gene names: JW0570, b0581, ybdK
Sequence domains: Glutamate-cysteine ligase family 2(GCS2)
Structure domains: Creatine Kinase; Chain A, domain 2

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 49.47Å b: 100.32Å c: 71.37Å
α: 90° β: 99.94° γ: 90°
R-values:
R R work R free
0.189 0.189 0.252
Expression system: Escherichia coli