› 1r6v

X-ray diffraction
1.7Å resolution

Crystal structure of fervidolysin from Fervidobacterium pennivorans, a keratinolytic enzyme related to subtilisin

Released:
DOI: 10.2210/pdb1r6v/pdb
PDB entry 1r6v coloured by chain, front view.
PDB entry 1r6v coloured by chain, side view.
PDB entry 1r6v coloured by chain, top view.
Source organism: Fervidobacterium pennivorans
Primary publication:
Crystal structure of fervidolysin from Fervidobacterium pennivorans, a keratinolytic enzyme related to subtilisin.
Kim JS, Kluskens LD, de Vos WM, Huber R, van der Oost J
J Mol Biol 335 787-97 (2004)
PMID: 14687574

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188097 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fervidolysin Chain: A
Molecule details ›
Chain: A
Length: 671 amino acids
Theoretical weight: 72 KDa
Source organism: Fervidobacterium pennivorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93LQ6 (Residues: 29-699; Coverage: 99%)
Gene name: fls
Sequence domains: Subtilase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P1
Unit cell:
a: 49.558Å b: 51.031Å c: 79.101Å
α: 93.9° β: 104.72° γ: 115.43°
R-values:
R R work R free
0.186 0.186 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Microbial keratinases and their prospective applications: an overview.
Gupta et al. (2006)
6 more

8 mentions without citation

Microbial enzymes catalyzing keratin degradation: Classification, structure, function.
Qiu et al. (2020)
7 more