PDBe 1r4i

X-ray diffraction
3.1Å resolution

Crystal Structure of Androgen Receptor DNA-Binding Domain Bound to a Direct Repeat Response Element

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Androgen receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 105 amino acids
Theoretical weight: 11.65 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15207 (Residues: 533-637; Coverage: 12%)
Gene names: Ar, Nr3c4
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*TP*CP*AP*AP*GP*AP*AP*CP*AP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.51 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*CP*TP*GP*TP*TP*CP*TP*TP*GP*AP*TP*GP*TP*TP*CP*TP*GP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3221
Unit cell:
a: 137.89Å b: 137.89Å c: 85.71Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.246 0.246 0.264
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided