PDBe 1r30

X-ray diffraction
3.4Å resolution

The Crystal Structure of Biotin Synthase, an S-Adenosylmethionine-Dependent Radical Enzyme

Released:

Function and Biology Details

Reaction catalysed:
Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Biotin synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 369 amino acids
Theoretical weight: 41.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P12996 (Residues: 2-346; Coverage: 100%)
Gene names: JW0758, b0775, bioB
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3121
Unit cell:
a: 155.69Å b: 155.69Å c: 90.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.256 0.256 0.3
Expression system: Escherichia coli