PDBe 1r2z

X-ray diffraction
1.63Å resolution

MutM (Fpg) bound to 5,6-dihydrouracil (DHU) containing DNA

Released:
Primary publication:
DNA lesion recognition by the bacterial repair enzyme MutM.
J. Biol. Chem. 278 51543-8 (2003)
PMID: 14525999

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Formamidopyrimidine-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 30.74 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P84131 (Residues: 1-274; Coverage: 100%)
Gene names: fpg, mutM
Sequence domains:
Structure domains:
5'-D(*AP*GP*GP*TP*AP*GP*AP*CP*CP*TP*GP*GP*AP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.69 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*TP*GP*CP*GP*TP*CP*CP*AP*(DHU)P*GP*TP*CP*TP*AP*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.6 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 44.591Å b: 94.226Å c: 102.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.233
Expression systems:
  • Escherichia coli
  • Not provided