› 1r0x

X-ray diffraction
2.2Å resolution

Cystic fibrosis transmembrane conductance regulator (CFTR) nucleotide-binding domain one (NBD1) with ATP

Released:
DOI: 10.2210/pdb1r0x/pdb
PDB entry 1r0x coloured by chain, front view.
PDB entry 1r0x coloured by chain, side view.
PDB entry 1r0x coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of nucleotide-binding domain 1 of the cystic fibrosis transmembrane conductance regulator.
Lewis HA, Buchanan SG, Burley SK, Conners K, Dickey M, Dorwart M, Fowler R, Gao X, Guggino WB, Hendrickson WA, Hunt JF, Kearins MC, Lorimer D, Maloney PC, Post KW, Rajashankar KR, Rutter ME, Sauder JM, Shriver S, Thibodeau PH, Thomas PJ, Zhang M, Zhao X, Emtage S
EMBO J 23 282-93 (2004)
PMID: 14685259

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-150714 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystic fibrosis transmembrane conductance regulator Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 286 amino acids
Theoretical weight: 32.1 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26361 (Residues: 389-673; Coverage: 19%)
Gene names: Abcc7, Cftr
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand ATP 4 x ATP
Ligand ACY 6 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P4212
Unit cell:
a: 173.292Å b: 173.292Å c: 110.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.211 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

62 review citations

The ABC protein turned chloride channel whose failure causes cystic fibrosis.
Gadsby et al. (2006)
61 more

11 mentions without citation

Structural changes of CFTR R region upon phosphorylation: a plastic platform for intramolecular and intermolecular interactions.
Bozoky et al. (2013)
10 more