PDBe 1r0o

X-ray diffraction
2.24Å resolution

Crystal Structure of the Heterodimeric Ecdysone Receptor DNA-binding Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Ecdysone receptor Chain: B
Molecule details ›
Chain: B
Length: 109 amino acids
Theoretical weight: 12.55 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P34021 (Residues: 256-364; Coverage: 12%)
Gene names: CG1765, EcR, NR1H1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
Protein ultraspiracle Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 10.09 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20153 (Residues: 94-179; Coverage: 17%)
Gene names: CG4380, Cf1, NR2B4, usp
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
Ecdysone Response Element Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.5 KDa
Ecdysone Response Element Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.49 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 50.37Å b: 59.88Å c: 113.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.232 0.27
Expression system: Escherichia coli BL21(DE3)