PDBe 1r0n

X-ray diffraction
2.6Å resolution

Crystal Structure of Heterodimeric Ecdsyone receptor DNA binding complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Ecdysone receptor Chain: B
Molecule details ›
Chain: B
Length: 109 amino acids
Theoretical weight: 12.55 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P34021 (Residues: 256-364; Coverage: 12%)
Gene names: CG1765, EcR, NR1H1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
Retinoic acid receptor RXR-alpha Chain: A
Molecule details ›
Chain: A
Length: 81 amino acids
Theoretical weight: 9.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19793 (Residues: 130-206; Coverage: 17%)
Gene names: NR2B1, RXRA
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
Ecdsyone Response Element Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.5 KDa
Ecdysone Response Element Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.49 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 55.62Å b: 60.26Å c: 115Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.233 0.292
Expression system: Escherichia coli BL21(DE3)