› 1qwn

X-ray diffraction
1.2Å resolution

GOLGI ALPHA-MANNOSIDASE II Covalent Intermediate Complex with 5-fluoro-gulosyl-fluoride

Released:
DOI: 10.2210/pdb1qwn/pdb
PDB entry 1qwn coloured by chain, front view.
PDB entry 1qwn coloured by chain, side view.
PDB entry 1qwn coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Insights into the mechanism of Drosophila melanogaster Golgi alpha-mannosidase II through the structural analysis of covalent reaction intermediates.
Numao S, Kuntz DA, Withers SG, Rose DR
J Biol Chem 278 48074-83 (2003)
PMID: 12960159

Function and Biology Details

Reaction catalysed: 
Man(5)GlcNAc(3)-[protein] + 2 H(2)O = Man(3)GlcNAc(3)-[protein] + 2 alpha-D-mannopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

5 bound ligands:
Ligand NAG 1 x NAG
Ligand ZN 1 x ZN
Ligand GUL 1 x GUL
Ligand TRS 1 x TRS
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 69.019Å b: 110.019Å c: 138.961Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.175 0.192
Expression system: Drosophila melanogaster

Quick links

Citations

6 review citations

Mechanistic insights into glycosidase chemistry.
Vocadlo et al. (2008)
5 more

3 mentions without citation

Crystallographic refinement of ligand complexes.
Kleywegt GJ. (2007)
2 more