PDBe 1qw7

X-ray diffraction
1.9Å resolution

Structure of an Engineered Organophosphorous Hydrolase with Increased Activity Toward Hydrolysis of Phosphothiolate Bonds

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Parathion hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 336 amino acids
Theoretical weight: 36.39 KDa
Source organism: Brevundimonas diminuta
Expression system: Escherichia coli
UniProt:
  • Best match: P0A434 (Residues: 30-365)
Gene name: opd

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 126.719Å b: 89.879Å c: 68.337Å
α: 90° β: 91.73° γ: 90°
R-values:
R R work R free
0.179 0.179 0.23
Expression system: Escherichia coli