EC 126.96.36.199: Deoxyribonuclease IV
Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.
- The enzyme is an apurinic/apyrimidinic (AP) site endonuclease that primes DNA repair synthesis at AP sites.
- It specifically cleaves the DNA backbone at AP sites and also removes 3' DNA-blocking groups such as 3' phosphates, 3' phosphoglycolates, and 3' alpha,beta-unsaturated aldehydes that arise from oxidative base damage and the activity of combined glycosylase/lyase enzymes.
- It is also the only known repair enzyme that is able to cleave the DNA backbone 5' of the oxidative lesion alpha-deoxyadenosine.
- The enzyme has a strong preference for single-stranded DNA.
- Formerly EC 188.8.131.52.
- E. coli endonuclease IV
- Escherichia coli endonuclease II
- Endodeoxyribonuclease IV (phage T(4)-induced)
- Endonuclease II
- Endodeoxyribonuclease IV (phage T4-induced)
- Endonuclease IV
- Deoxyribonuclease IV (phage T4-induced)
- Nfo (gene name)
- not assigned
Protein family (Pfam)
Domain description: Xylose isomerase-like TIM barrel
Domain description: AP endonuclease 2
Domain description: AP endonuclease 2, zinc binding site
Domain description: Xylose isomerase-like, TIM barrel domain
Domain description: Xylose isomerase-like superfamily
Class: Alpha Beta
Architecture: Alpha-Beta Barrel
Topology: TIM Barrel
Homology: Divalent-metal-dependent TIM barrel enzymesOccurring in:
Class: Alpha and beta proteins (a/b)
Fold: TIM beta/alpha-barrel
Superfamily: Xylose isomerase-likeOccurring in: