PDBe 1qum

X-ray diffraction
1.55Å resolution

CRYSTAL STRUCTURE OF ESCHERICHIA COLI ENDONUCLEASE IV IN COMPLEX WITH DAMAGED DNA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Endonuclease 4 Chain: A
Molecule details ›
Chain: A
Length: 285 amino acids
Theoretical weight: 31.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Best match: P0A6C1 (Residues: 1-285)
Gene names: JW2146, b2159, nfo
5'-D(*CP*GP*TP*CP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 6 nucleotides
Theoretical weight: 1.79 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*(3DR)P*CP*GP*AP*CP*GP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 7 nucleotides
Theoretical weight: 2 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*TP*CP*GP*TP*CP*GP*GP*GP*GP*AP*CP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 13 nucleotides
Theoretical weight: 4.01 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: C2
Unit cell:
a: 133.12Å b: 58.727Å c: 51.146Å
α: 90° β: 94.97° γ: 90°
R-values:
R R work R free
0.203 0.203 0.254
Expression systems:
  • Escherichia coli
  • Not provided