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X-RAY STRUCTURE OF THE DNA-ECO RI ENDONUCLEASE COMPLEXES WITH AN R145K MUTATION AT 2.7 A

X-ray diffraction
2.5Å resolution
PDB entry 1qrh coloured by chain, front view.
PDB entry 1qrh coloured by chain, side view.
PDB entry 1qrh coloured by chain, top view.
Source organism: Escherichia coli
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct DNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Type II restriction enzyme EcoRI and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 23718.13 Å2
Buried surface area: 9681.87 Å2
Dissociation area: 3,336.22 Å2
Dissociation energy (ΔGdiss): 21.8 kcal/mol
Dissociation entropy (TΔSdiss): 14.39 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-114464

Macromolecules

Type II restriction enzyme EcoRI
Chain: A
Length: 261 amino acids
Theoretical weight: 29.14 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P00642 (Residues: 17-277; Coverage: 94%)
Gene name: ecoRIR
Pfam: Restriction endonuclease EcoRI
InterPro:
CATH: Eco RI Endonuclease, subunit A
SCOP: Restriction endonuclease EcoRI
Type II restriction enzyme EcoRI in PDB entry 1qrh, assembly 1, front view.
Type II restriction enzyme EcoRI in PDB entry 1qrh, assembly 1, side view.
Type II restriction enzyme EcoRI in PDB entry 1qrh, assembly 1, top view.
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DNA chain M
Name: 5'-(TP*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G*)-3'
Representative chains: M
Source organism: Escherichia coli [562]
Expression system: Not provided
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
5'-(TP*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G*)-3' in PDB entry 1qrh, assembly 1, front view.
5'-(TP*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G*)-3' in PDB entry 1qrh, assembly 1, side view.
5'-(TP*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G*)-3' in PDB entry 1qrh, assembly 1, top view.
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