PDBe 1qrh

X-ray diffraction
2.5Å resolution

X-RAY STRUCTURE OF THE DNA-ECO RI ENDONUCLEASE COMPLEXES WITH AN R145K MUTATION AT 2.7 A

Released:
Entry authors: Choi J, Kim Y, Greene P, Hager P, Rosenberg JM

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type-2 restriction enzyme EcoRI Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 29.14 KDa
Source organism: Escherichia coli
UniProt:
  • Best match: P00642 (Residues: 17-277)
Gene name: ecoRIR
5'-(TP*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G*)-3' Chain: M
Molecule details ›
Chain: M
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P321
Unit cell:
a: 118.4Å b: 118.4Å c: 49.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.161 not available
Expression system: Not provided