PDBe 1qp9

X-ray diffraction
2.8Å resolution

STRUCTURE OF HAP1-PC7 COMPLEXED TO THE UAS OF CYC7

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Heme-responsive zinc finger transcription factor HAP1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 76 amino acids
Theoretical weight: 9.09 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CS82 (Residues: 55-130; Coverage: 5%)
Gene names: CYP1, HAP1
Sequence domains: Fungal Zn(2)-Cys(6) binuclear cluster domain
Structure domains: CD2-Gal4
DNA (5'-D(*AP*CP*GP*CP*TP*AP*TP*TP*AP*TP*CP*GP*CP*TP*AP*TP*TP*AP*GP*T)-3') Chains: E, G
Molecule details ›
Chains: E, G
Length: 20 nucleotides
Theoretical weight: 6.1 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*AP*CP*TP*AP*AP*TP*AP*GP*CP*GP*AP*TP*AP*AP*TP*AP*GP*CP*GP*T)-3') Chains: F, H
Molecule details ›
Chains: F, H
Length: 20 nucleotides
Theoretical weight: 6.17 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 85.9Å b: 90.9Å c: 96.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.255 0.294
Expression systems:
  • Escherichia coli
  • Not provided