PDBe 1qmz

X-ray diffraction
2.2Å resolution

PHOSPHORYLATED CDK2-CYCLYIN A-SUBSTRATE PEPTIDE COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
SUBSTRATE PEPTIDE Chains: E, F
Molecule details ›
Chains: E, F
Length: 7 amino acids
Theoretical weight: 836 Da
Source organism: Homo sapiens
Expression system: Not provided
Cyclin-A2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 259 amino acids
Theoretical weight: 29.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20248 (Residues: 174-432; Coverage: 60%)
Gene names: CCN1, CCNA, CCNA2
Sequence domains:
Structure domains: Cyclin A; domain 1
Cyclin-dependent kinase 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 299 amino acids
Theoretical weight: 34.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P24941 (Residues: 1-298; Coverage: 100%)
Gene names: CDK2, CDKN2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21212
Unit cell:
a: 152.6Å b: 163.7Å c: 73.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.28
Expression systems:
  • Not provided
  • Escherichia coli