PDBe 1qk4

X-ray diffraction
1.9Å resolution

TOXOPLASMA GONDII HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE IMP COMPLEX

Released:

Function and Biology Details

Reactions catalysed:
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate. 
XMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine-guanine-xanthine phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 233 amino acids
Theoretical weight: 26.71 KDa
Source organism: Toxoplasma gondii RH
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q26997 (Residues: 1-230; Coverage: 100%)
Gene name: HPRT
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 84.537Å b: 102.445Å c: 108.825Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.238
Expression system: Escherichia coli BL21(DE3)