PDBe 1qh9

X-ray diffraction
2.5Å resolution

ENZYME-PRODUCT COMPLEX OF L-2-HALOACID DEHALOGENASE

Released:
Source organism: Pseudomonas sp. YL
Entry authors: Li Y-F, Hata Y, Fujii T, Kurihara T, Esaki N

Function and Biology Details

Reaction catalysed:
(S)-2-haloacid + H(2)O = (R)-2-hydroxyacid + halide. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-2-haloacid dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 26.21 KDa
Source organism: Pseudomonas sp. YL
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53464 (Residues: 1-232; Coverage: 100%)
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2
Unit cell:
a: 91.81Å b: 62.76Å c: 51.38Å
α: 90° β: 123.07° γ: 90°
R-values:
R R work R free
0.194 0.194 0.287
Expression system: Escherichia coli