PDBe 1qd6

X-ray diffraction
2.1Å resolution

OUTER MEMBRANE PHOSPHOLIPASE A FROM ESCHERICHIA COLI

Released:

Function and Biology Details

Reactions catalysed:
Phosphatidylcholine + H(2)O = 2-acylglycerophosphocholine + a carboxylate
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phospholipase A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 13 amino acids
Theoretical weight: 1.4 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
UniProt:
  • Canonical: P0A921 (Residues: 33-45; Coverage: 5%)
Gene names: JW3794, b3821, pldA
Phospholipase A1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 240 amino acids
Theoretical weight: 27.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A921 (Residues: 50-289; Coverage: 89%)
Gene names: JW3794, b3821, pldA
Structure domains: Outer membrane phospholipase A (OMPLA)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID2
Spacegroup: P212121
Unit cell:
a: 80.067Å b: 84.038Å c: 95.245Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.283
Expression systems:
  • Not provided
  • Escherichia coli