1qbk

X-ray diffraction
3Å resolution

STRUCTURE OF THE KARYOPHERIN BETA2-RAN GPPNHP NUCLEAR TRANSPORT COMPLEX

Released:
Source organism: Homo sapiens
Primary publication:
Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp.
Nature 399 230-7 (1999)
PMID: 10353245

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
PDBe Complex ID:
PDB-CPX-158725 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transportin-1 Chain: B
Molecule details ›
Chain: B
Length: 890 amino acids
Theoretical weight: 102.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92973 (Residues: 9-898; Coverage: 99%)
Gene names: KPNB2, MIP1, TNPO1, TRN
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
GTP-binding nuclear protein Ran Chain: C
Molecule details ›
Chain: C
Length: 216 amino acids
Theoretical weight: 24.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62826 (Residues: 1-216; Coverage: 100%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3221
Unit cell:
a: 133.11Å b: 133.11Å c: 138.41Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.267 0.267 0.315
Expression system: Escherichia coli