PDBe 1qam

X-ray diffraction
2.2Å resolution

THE STRUCTURE OF THE RRNA METHYLTRANSFERASE ERMC': IMPLICATIONS FOR THE REACTION MECHANISM

Released:

Function and Biology Details

Reaction catalysed:
2 S-adenosyl-L-methionine + adenine(2085) in 23S rRNA = 2 S-adenosyl-L- homocysteine + N(6)-dimethyladenine(2085) in 23S rRNA. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
rRNA adenine N-6-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 28.96 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P13956 (Residues: 1-244; Coverage: 100%)
Gene name: ermC'
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 81.38Å b: 81.38Å c: 122.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.251
Expression system: Escherichia coli