PDBe 1q6x

X-ray diffraction
2.5Å resolution

Crystal structure of rat choline acetyltransferase

Released:

Function and Biology Details

Reaction catalysed:
Acetyl-CoA + choline = CoA + O-acetylcholine. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Choline O-acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 644 amino acids
Theoretical weight: 72.48 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32738 (Residues: 1-636; Coverage: 99%)
Gene name: Chat
Sequence domains: Choline/Carnitine o-acyltransferase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 84.701Å b: 78.882Å c: 139.364Å
α: 90° β: 98.38° γ: 90°
R-values:
R R work R free
0.226 0.223 0.251
Expression system: Escherichia coli