PDBe 1q3n

X-ray diffraction
2.7Å resolution

Crystal structure of KDO8P synthase in its binary complex with substrate PEP

Released:

Function and Biology Details

Reaction catalysed:
Phosphoenolpyruvate + D-arabinose 5-phosphate + H(2)O = 3-deoxy-D-manno- octulosonate 8-phosphate + phosphate. 
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydro-3-deoxyphosphooctonate aldolase Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 30.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A715 (Residues: 1-284; Coverage: 100%)
Gene names: JW1206, b1215, kdsA
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: I23
Unit cell:
a: 118.441Å b: 118.441Å c: 118.441Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.235 0.268
Expression system: Escherichia coli