PDBe 1q3c

X-ray diffraction
2.3Å resolution

Crystal structure of the DNA repair enzyme endonuclease-VIII (Nei) from E. coli: The E2A mutant at 2.3 resolution.

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease 8 Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 29.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P50465 (Residues: 2-263; Coverage: 100%)
Gene names: JW0704, b0714, nei
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: I222
Unit cell:
a: 57.779Å b: 81.067Å c: 169.935Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.304
Expression system: Escherichia coli