PDBe 1q18

X-ray diffraction
2.36Å resolution

Crystal structure of E.coli glucokinase (Glk)

Released:

Function and Biology Details

Reaction catalysed:
ATP + D-glucose = ADP + D-glucose 6-phosphate. 
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucokinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 332 amino acids
Theoretical weight: 36.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6V9 (Residues: 2-10, 12-321; Coverage: 99%)
Gene names: ECs3268, Z3654, glk
Sequence domains: Glucokinase
Structure domains:

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P43212
Unit cell:
a: 81.474Å b: 81.474Å c: 234.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.267
Expression system: Escherichia coli BL21(DE3)