PDBe 1q0z

X-ray diffraction
1.95Å resolution

Crystal structure of aclacinomycin methylesterase (RdmC) with bound product analogue, 10-decarboxymethylaclacinomycin A (DcmA)

Released:

Function and Biology Details

Reaction catalysed:
Aclacinomycin T + H(2)O = 15-demethylaclacinomycin T + methanol. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aclacinomycin methylesterase RdmC Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 31.83 KDa
Source organism: Streptomyces purpurascens
Expression system: Streptomyces lividans TK24
UniProt:
  • Canonical: Q54528 (Residues: 1-298; Coverage: 100%)
Gene name: rdmC
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 38.106Å b: 84.875Å c: 44.389Å
α: 90° β: 99.99° γ: 90°
R-values:
R R work R free
0.159 0.157 0.185
Expression system: Streptomyces lividans TK24