PDBe 1pyy

X-ray diffraction
2.42Å resolution

Double mutant PBP2x T338A/M339F from Streptococcus pneumoniae strain R6 at 2.4 A resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-binding protein 2X Chain: A
Molecule details ›
Chain: A
Length: 702 amino acids
Theoretical weight: 76.79 KDa
Source organism: Streptococcus pneumoniae R6
Expression system: Escherichia coli
UniProt:
  • Canonical: P59676 (Residues: 49-750; Coverage: 94%)
Gene names: pbpX, spr0304
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 128.224Å b: 64.746Å c: 146.974Å
α: 90° β: 118.89° γ: 90°
R-values:
R R work R free
0.244 0.218 0.244
Expression system: Escherichia coli