PDBe 1pyi

X-ray diffraction
3.2Å resolution

CRYSTAL STRUCTURE OF A PPR1-DNA COMPLEX: DNA RECOGNITION BY PROTEINS CONTAINING A ZN2CYS6 BINUCLEAR CLUSTER

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Pyrimidine pathway regulatory protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 10.96 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Best match: P07272 (Residues: 29-123)
Gene names: PPR1, YLR014C
DNA (5'-D(*TP*CP*GP*GP*CP*AP*AP*TP*TP*GP*CP*CP*GP*A)-3') Chains: D, E
Molecule details ›
Chains: D, E
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I222
Unit cell:
a: 53.8Å b: 92.6Å c: 174.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 not available 0.33
Expression systems:
  • Escherichia coli
  • Not provided