PDBe 1pxv

X-ray diffraction
1.8Å resolution

The staphostatin-staphopain complex: a forward binding inhibitor in complex with its target cysteine protease

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Staphopain B Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 21.06 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C1S6 (Residues: 218-393; Coverage: 49%)
Gene name: sspB
Sequence domains: Staphopain peptidase C47
Structure domains: Cysteine proteinases
Staphostatin B Chains: C, D
Molecule details ›
Chains: C, D
Length: 111 amino acids
Theoretical weight: 13.07 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EYW6 (Residues: 1-109; Coverage: 100%)
Gene names: SAOUHSC_00986, sspC
Sequence domains: Staphostatin B
Structure domains: beta-Barrel protease inhibitors

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 73.485Å b: 94.966Å c: 110.927Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.221
Expression system: Escherichia coli