› 1px8

X-ray diffraction
2.4Å resolution

Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase

Released:
DOI: 10.2210/pdb1px8/pdb
PDB entry 1px8 coloured by chain, front view.
PDB entry 1px8 coloured by chain, side view.
PDB entry 1px8 coloured by chain, top view.
Source organism: Thermoanaerobacterium saccharolyticum
Primary publication:
Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase.
Yang JK, Yoon HJ, Ahn HJ, Lee BI, Pedelacq JD, Liong EC, Berendzen J, Laivenieks M, Vieille C, Zeikus GJ, Vocadlo DJ, Withers SG, Suh SW
J Mol Biol 335 155-65 (2004)
PMID: 14659747

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153255 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-xylosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 500 amino acids
Theoretical weight: 58.74 KDa
Source organism: Thermoanaerobacterium saccharolyticum
Expression system: Escherichia coli
UniProt:
  • Canonical: P36906 (Residues: 1-500; Coverage: 100%)
Gene name: xynB
Sequence domains: Glycosyl hydrolases family 39
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand XYP 2 x XYP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: P43212
Unit cell:
a: 92.719Å b: 92.719Å c: 241.296Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.224 0.271
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Microbial xylanases: engineering, production and industrial applications.
Juturu et al. (2012)
5 more

12 mentions without citation

β-Xylosidases: Structural Diversity, Catalytic Mechanism, and Inhibition by Monosaccharides.
Rohman et al. (2019)
11 more