PDBe 1pvu

X-ray diffraction
2.4Å resolution

THE CRYSTAL STRUCTURE OF PVUII ENDONUCLEASE REVEALS EXTENSIVE STRUCTURAL HOMOLOGIES TO ECORV

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-2 restriction enzyme PvuII Chains: A, B
Molecule details ›
Chains: A, B
Length: 156 amino acids
Theoretical weight: 18.24 KDa
Source organism: Proteus vulgaris
Expression system: Not provided
UniProt:
  • Canonical: P23657 (Residues: 2-157; Coverage: 99%)
Gene name: pvuIIR
Sequence domains: Restriction endonuclease PvuII
Structure domains: PVUII Endonuclease, subunit A

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 84.65Å b: 106.46Å c: 47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 not available
Expression system: Not provided