PDBe 1puf

X-ray diffraction
1.9Å resolution

Crystal Structure of HoxA9 and Pbx1 homeodomains bound to DNA

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Homeobox protein Hox-A9 Chain: A
Molecule details ›
Chain: A
Length: 77 amino acids
Theoretical weight: 9.65 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P09631 (Residues: 193-269; Coverage: 28%)
Gene names: Hox-1.7, Hoxa-9, Hoxa9
Sequence domains: Homeobox domain
Structure domains: Homeodomain-like
Pre-B-cell leukemia transcription factor 1 Chain: B
Molecule details ›
Chain: B
Length: 73 amino acids
Theoretical weight: 8.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40424 (Residues: 233-305; Coverage: 17%)
Gene names: PBX1, PRL
Sequence domains: Homeobox domain
Structure domains: Homeodomain-like
5'-D(*AP*CP*TP*CP*TP*AP*TP*GP*AP*TP*TP*TP*AP*CP*GP*AP*CP*GP*CP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.08 KDa
5'-D(*TP*AP*GP*CP*GP*TP*CP*GP*TP*AP*AP*AP*TP*CP*AP*TP*AP*GP*AP*G)-3' Chain: E
Molecule details ›
Chain: E
Length: 20 nucleotides
Theoretical weight: 6.18 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 60.769Å b: 114.853Å c: 108.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.268
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)