PDBe 1pud

X-ray diffraction
1.85Å resolution

TRNA-GUANINE TRANSGLYCOSYLASE

Released:

Function and Biology Details

Reaction catalysed:
(1) Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine. (2) Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7- carbaguanine(34) in tRNA + guanine. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Queuine tRNA-ribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 42.93 KDa
Source organism: Zymomonas mobilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P28720 (Residues: 1-386; Coverage: 100%)
Gene names: ZMO0363, tgt
Sequence domains: Queuine tRNA-ribosyltransferase
Structure domains: tRNA-guanine (tRNA-G) transglycosylase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE
Spacegroup: C2
Unit cell:
a: 92.1Å b: 65.1Å c: 71.9Å
α: 90° β: 97.5° γ: 90°
R-values:
R R work R free
0.19 0.19 0.214
Expression system: Escherichia coli