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EF-hands at atomic resolution: the structure of human psoriasin (S100A7) solved by MAD phasing.

Brodersen DE, Etzerodt M, Madsen P, Celis JE, Thøgersen HC, Nyborg J, Kjeldgaard M
Structure 6 477-89 (1998)
Cited: 70 times
EuropePMC logo PMID: 9562557

Abstract

Background

The S100 family consists of small acidic proteins, belonging to the EF-hand class of calcium-binding proteins. They are primarily regulatory proteins, involved in cell growth, cell structure regulation and signal transduction. Psoriasin (S100A7) is an 11.7 kDa protein that is highly upregulated in the epidermis of patients suffering from the chronic skin disease psoriasis. Although its exact function is not known, psoriasin is believed to participate in the biochemical response which follows transient changes in the cellular Ca2+ concentration.

Results

The three-dimensional structure of holmium-substituted psoriasin has been determined by multiple anomalous wavelength dispersion (MAD) phasing and refined to atomic resolution (1.05 A). The structure represents the most accurately determined structure of a calcium-binding protein. Although the overall structure of psoriasin is similar to those of other S100 proteins, several important differences exist, mainly in the N-terminal EF-hand motif that contains a distorted loop and lacks a crucial calcium-binding residue. It is these minor differences that may account for the different specificities among members of this family.

Conclusion

The structure of human psoriasin reveals that this protein, in contrast to other S100 proteins with known structure, is not likely to strongly bind more than one calcium ion per monomer. The present study contradicts the idea that calcium binding induces large changes in conformation, as suggested by previously determined structures of apo forms of S100 proteins. The substitution of Ca2+ ions in EF-hands by lanthanide ions may provide a general vehicle for structure determination of S100 proteins by means of MAD phasing.

Reviews - 1psr mentioned but not cited (2)

  1. Zooming into the Dark Side of Human Annexin-S100 Complexes: Dynamic Alliance of Flexible Partners. Weisz J, Uversky VN. Int J Mol Sci 21 E5879 (2020)
  2. S100 Proteins as Novel Therapeutic Targets in Psoriasis and Other Autoimmune Diseases. Kurpet K, Chwatko G. Molecules 27 6640 (2022)

Articles - 1psr mentioned but not cited (12)

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Reviews citing this publication (21)

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Articles citing this publication (35)

  1. Structural basis of the Ca(2+)-dependent association between S100C (S100A11) and its target, the N-terminal part of annexin I. Réty S, Osterloh D, Arié JP, Tabaries S, Seeman J, Russo-Marie F, Gerke V, Lewit-Bentley A. Structure 8 175-184 (2000)
  2. A powerful combinatorial screen to identify high-affinity terbium(III)-binding peptides. Nitz M, Franz KJ, Maglathlin RL, Imperiali B. Chembiochem 4 272-276 (2003)
  3. Crystal structures of S100A6 in the Ca(2+)-free and Ca(2+)-bound states: the calcium sensor mechanism of S100 proteins revealed at atomic resolution. Otterbein LR, Kordowska J, Witte-Hoffmann C, Wang CL, Dominguez R. Structure 10 557-567 (2002)
  4. Betaine-homocysteine methyltransferase: zinc in a distorted barrel. Evans JC, Huddler DP, Jiracek J, Castro C, Millian NS, Garrow TA, Ludwig ML. Structure 10 1159-1171 (2002)
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  6. The crystal structure of human MRP14 (S100A9), a Ca(2+)-dependent regulator protein in inflammatory process. Itou H, Yao M, Fujita I, Watanabe N, Suzuki M, Nishihira J, Tanaka I. J. Mol. Biol. 316 265-276 (2002)
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  13. High resolution solution structure of apo calcyclin and structural variations in the S100 family of calcium-binding proteins. Mäler L, Potts BC, Chazin WJ. J. Biomol. NMR 13 233-247 (1999)
  14. The human antimicrobial protein psoriasin acts by permeabilization of bacterial membranes. Michalek M, Gelhaus C, Hecht O, Podschun R, Schröder JM, Leippe M, Grötzinger J. Dev. Comp. Immunol. 33 740-746 (2009)
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  18. Crystal structure of the EF-hand parvalbumin at atomic resolution (0.91 A) and at low temperature (100 K). Evidence for conformational multistates within the hydrophobic core. Declercq JP, Evrard C, Lamzin V, Parello J. Protein Sci. 8 2194-2204 (1999)
  19. The 0.93A crystal structure of sphericase: a calcium-loaded serine protease from Bacillus sphaericus. Almog O, González A, Klein D, Greenblatt HM, Braun S, Shoham G. J. Mol. Biol. 332 1071-1082 (2003)
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  21. Observation of microsecond time-scale protein dynamics in the presence of Ln3+ ions: application to the N-terminal domain of cardiac troponin C. Eichmüller C, Skrynnikov NR. J Biomol NMR 37 79-95 (2007)
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  24. S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL-6 secretion through IκB/NF-κB signalling. Son ED, Kim HJ, Kim KH, Bin BH, Bae IH, Lim KM, Yu SJ, Cho EG, Lee TR. Exp. Dermatol. 25 636-641 (2016)
  25. Structural and functional characterization of human Iba proteins. Schulze JO, Quedenau C, Roske Y, Adam T, Schüler H, Behlke J, Turnbull AP, Sievert V, Scheich C, Mueller U, Heinemann U, Büssow K. FEBS J. 275 4627-4640 (2008)
  26. Comprehensive evaluation of genetic variation in S100A7 suggests an association with the occurrence of allergic rhinitis. Bryborn M, Halldén C, Säll T, Adner M, Cardell LO. Respir. Res. 9 29 (2008)
  27. X-ray structures of the microglia/macrophage-specific protein Iba1 from human and mouse demonstrate novel molecular conformation change induced by calcium binding. Yamada M, Ohsawa K, Imai Y, Kohsaka S, Kamitori S. J. Mol. Biol. 364 449-457 (2006)
  28. Biochemical and Functional Evaluation of the Intramolecular Disulfide Bonds in the Zinc-Chelating Antimicrobial Protein Human S100A7 (Psoriasin). Cunden LS, Brophy MB, Rodriguez GE, Flaxman HA, Nolan EM. Biochemistry 56 5726-5738 (2017)
  29. Structural characterization of human S100A16, a low-affinity calcium binder. Babini E, Bertini I, Borsi V, Calderone V, Hu X, Luchinat C, Parigi G. J. Biol. Inorg. Chem. 16 243-256 (2011)
  30. Bioinorganic Explorations of Zn(II) Sequestration by Human S100 Host-Defense Proteins. Cunden LS, Nolan EM. Biochemistry 57 1673-1680 (2018)
  31. An orthologue of the host-defense protein psoriasin (S100A7) is expressed in frog skin. Matthijs S, Hernalsteens JP, Roelants K. Dev. Comp. Immunol. 67 395-403 (2017)
  32. Structural and functional characterization of a triple mutant form of S100A7 defective for Jab1 binding. West NR, Farnell B, Murray JI, Hof F, Watson PH, Boulanger MJ. Protein Sci. 18 2615-2623 (2009)
  33. Comparative analysis of the physical properties of murine and human S100A7: Insight into why zinc piracy is mediated by human but not murine S100A7. Harrison SA, Naretto A, Balakrishnan S, Perera YR, Chazin WJ. J Biol Chem 299 105292 (2023)
  34. Solving the crystal structure of human calcium-free S100Z: the siege and conquer of one of the last S100 family strongholds. Calderone V, Fragai M, Gallo G, Luchinat C. J. Biol. Inorg. Chem. 22 519-526 (2017)
  35. The Relationship between Mastitis and Antimicrobial Peptide S100A7 Expression in Dairy Goats. Yan Y, Zhu K, Liu H, Fan M, Zhao X, Pan M, Ma B, Wei Q. Vet Sci 10 653 (2023)


Related citations provided by authors (2)

  1. Crystallization and preliminary X-ray diffraction studies of psoriasin.. Nolsøe S, Thirup S, Etzerodt M, Thøgersen HC, Nyborg J Acta Crystallogr D Biol Crystallogr 53 119-21 (1997)
  2. Molecular Cloning, Occurrence, and Expression of a Novel Partially Secreted Protein "Psoriasin" that is Highly Up-Regulated in Psoriatic Skin. Madsen P, Rasmussen HH, Leffers H, Honore B, Dejgaard K, Olsen E, Kiil J, Walbum E, Andersen AH, Basse B, Lauridsen JB, Ratz GP, Celis A, Vandekerckhove J, Celis JE J. Invest. Dermatol. 97 701- (1991)

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