› 1pq2

X-ray diffraction
2.7Å resolution

Crystal Structure of Human Drug Metabolizing Cytochrome P450 2C8

Released:
DOI: 10.2210/pdb1pq2/pdb
PDB entry 1pq2 coloured by chain, front view.
PDB entry 1pq2 coloured by chain, side view.
PDB entry 1pq2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human microsomal cytochrome P450 2C8. Evidence for a peripheral fatty acid binding site.
Schoch GA, Yano JK, Wester MR, Griffin KJ, Stout CD, Johnson EF
J Biol Chem 279 9497-503 (2004)
PMID: 14676196

Function and Biology Details

Reaction catalysed: 
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 2C8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 476 amino acids
Theoretical weight: 54.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10632 (Residues: 28-490; Coverage: 95%)
Gene name: CYP2C8
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
2 bound ligands:
Ligand PO4 1 x PO4
Ligand PLM 2 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 105.988Å b: 137.405Å c: 97.322Å
α: 90° β: 112.51° γ: 90°
R-values:
R R work R free
0.249 0.248 0.288
Expression system: Escherichia coli

Quick links

Citations

43 review citations

Polymorphism of human cytochrome P450 enzymes and its clinical impact.
Zhou et al. (2009)
42 more

38 mentions without citation

Cytochrome P450: Polymorphisms and Roles in Cancer, Diabetes and Atherosclerosis
Elfaki et al. (2018)
37 more