› 1ppm

X-ray diffraction
1.7Å resolution

CRYSTALLOGRAPHIC ANALYSIS OF TRANSITION-STATE MIMICS BOUND TO PENICILLOPEPSIN: PHOSPHORUS-CONTAINING PEPTIDE ANALOGUES

Released:
DOI: 10.2210/pdb1ppm/pdb
PDB entry 1ppm coloured by chain, front view.
PDB entry 1ppm coloured by chain, side view.
PDB entry 1ppm coloured by chain, top view.
Source organism: Penicillium janthinellum
Primary publication:
Crystallographic analysis of transition-state mimics bound to penicillopepsin: phosphorus-containing peptide analogues.
Fraser ME, Strynadka NC, Bartlett PA, Hanson JE, James MN
Biochemistry 31 5201-14 (1992)
PMID: 1606144

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with broad specificity similar to that of pepsin A, preferring hydrophobic residues at P1 and P1', but also cleaving 20-Gly-|-Glu-21 in the B chain of insulin. Clots milk, and activates trypsinogen.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillopepsin-1 Chain: E
Molecule details ›
Chain: E
Length: 323 amino acids
Theoretical weight: 33.47 KDa
Source organism: Penicillium janthinellum
Expression system: Not provided
UniProt:
  • Canonical: P00798 (Residues: 1-323; Coverage: 100%)
Sequence domains: Eukaryotic aspartyl protease
Structure domains: Acid Proteases

Ligands and Environments

4 bound ligands:
Ligand 0P1 1 x 0P1
Ligand MAN 1 x MAN
Ligand ARA 1 x ARA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 97.4Å b: 46.59Å c: 66.29Å
α: 90° β: 115.7° γ: 90°
R-values:
R R work R free
0.15 0.15 not available
Expression system: Not provided

Quick links

Citations

3 review citations

"Strong" hydrogen bonds in chemistry and biology.
Perrin et al. (1997)
2 more

17 mentions without citation

Low barrier hydrogen bonds in protein structure and function.
Kemp et al. (2021)
16 more