PDBe 1pm5

X-ray diffraction
1.95Å resolution

Crystal structure of wild type Lactococcus lactis Fpg complexed to a tetrahydrofuran containing DNA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Formamidopyrimidine-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 31.12 KDa
Source organism: Lactococcus lactis subsp. cremoris
Expression system: Escherichia coli
UniProt:
  • Best match: P42371 (Residues: 2-273)
Gene names: fpg, mutM
DNA (5'-D(*GP*CP*GP*AP*GP*AP*AP*AP*CP*AP*AP*AP*GP*A)-3') Chain: E
Molecule details ›
Chain: E
Length: 14 nucleotides
Theoretical weight: 4.36 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*TP*CP*TP*TP*TP*(3DR)P*TP*TP*TP*CP*TP*CP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 14 nucleotides
Theoretical weight: 4.05 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P41212
Unit cell:
a: 91.784Å b: 91.784Å c: 141.792Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.216
Expression systems:
  • Escherichia coli
  • Not provided