› 1plg

X-ray diffraction
2.8Å resolution

EVIDENCE FOR THE EXTENDED HELICAL NATURE OF POLYSACCHARIDE EPITOPES. THE 2.8 ANGSTROMS RESOLUTION STRUCTURE AND THERMODYNAMICS OF LIGAND BINDING OF AN ANTIGEN BINDING FRAGMENT SPECIFIC FOR ALPHA-(2->8)-POLYSIALIC ACID

Released:
DOI: 10.2210/pdb1plg/pdb
PDB entry 1plg coloured by chain, front view.
PDB entry 1plg coloured by chain, side view.
PDB entry 1plg coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Evidence for the extended helical nature of polysaccharide epitopes. The 2.8 A resolution structure and thermodynamics of ligand binding of an antigen binding fragment specific for alpha-(2-->8)-polysialic acid.
Evans SV, Sigurskjold BW, Jennings HJ, Brisson JR, To R, Tse WC, Altman E, Frosch M, Weisgerber C, Kratzin HD
Biochemistry 34 6737-44 (1995)
PMID: 7538787

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106863 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.74 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: A2NHM3 (Residues: 1-215; Coverage: 98%)
Gene names: Igk-C, Igkc
Sequence domains:
Structure domains: Immunoglobulins
Ig gamma-2A chain C region, membrane-bound form Chain: H
Molecule details ›
Chain: H
Length: 215 amino acids
Theoretical weight: 23.02 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01865 (Residues: 1-97; Coverage: 24%)
Gene name: Igh-1a
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I222
Unit cell:
a: 79.14Å b: 91.21Å c: 141.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.164 not available

Quick links

Citations

13 review citations

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
12 more

12 mentions without citation

Tailoring in vitro evolution for protein affinity or stability.
Jermutus et al. (2001)
11 more