PDBe 1pk0

X-ray diffraction
3.3Å resolution

Crystal Structure of the EF3-CaM complexed with PMEApp

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-2 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 147 amino acids
Theoretical weight: 16.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DP24 (Residues: 2-148; Coverage: 99%)
Gene names: CALM2, CAM2, CAMB
Structure domains: EF-hand
Calmodulin-sensitive adenylate cyclase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 507 amino acids
Theoretical weight: 58.44 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: P40136 (Residues: 292-798; Coverage: 66%)
Gene names: BXA0141, GBAA_pXO1_0142, cya, pXO1-122
Sequence domains: Anthrax toxin LF subunit
Structure domains: Adenylylcyclase toxin superfamily

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 116.263Å b: 165.759Å c: 342.409Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.264 0.302
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli