PDBe 1pjq

X-ray diffraction
2.21Å resolution

Structure and function of CysG, the multifunctional methyltransferase/dehydrogenase/ferrochelatase for siroheme synthesis


Function and Biology Details

Reactions catalysed:
Siroheme + 2 H(+) = sirohydrochlorin + Fe(2+). 
Precorrin-2 + NAD(+) = sirohydrochlorin + NADH. 
(1) S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L- homocysteine + precorrin-1. (2) S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 60.734Å b: 121.493Å c: 130.79Å
α: 90° β: 90° γ: 90°
R R work R free
0.235 0.235 0.273
Expression system: Salmonella enterica