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Complex between the Lactococcus lactis Fpg and an abasic site containing DNA.

X-ray diffraction
1.9Å resolution
PDB entry 1pjj coloured by chain, front view.
PDB entry 1pjj coloured by chain, side view.
PDB entry 1pjj coloured by chain, top view.
Source organism: Lactococcus lactis
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Formamidopyrimidine-DNA glycosylase and DNA
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Multimeric state: hetero trimer
Accessible surface area: 16006.28 Å2
Buried surface area: 4572.73 Å2
Dissociation area: 1,262.17 Å2
Dissociation energy (ΔGdiss): 14.42 kcal/mol
Dissociation entropy (TΔSdiss): 11.56 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-114392

Macromolecules

Formamidopyrimidine-DNA glycosylase
Chain: A
Length: 271 amino acids
Theoretical weight: 31.08 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42371 (Residues: 2-273; Coverage: 99%)
Gene names: fpg, mutM
Pfam:
InterPro:
CATH:
SCOP:
Formamidopyrimidine-DNA glycosylase in PDB entry 1pjj, assembly 1, front view.
Formamidopyrimidine-DNA glycosylase in PDB entry 1pjj, assembly 1, side view.
Formamidopyrimidine-DNA glycosylase in PDB entry 1pjj, assembly 1, top view.
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DNA chain D
Name: DNA (5'-D(*CP*TP*CP*TP*TP*TP*(3DR)P*TP*TP*TP*CP*TP*CP*G)-3')
Representative chains: D
Source organism: Lactococcus lactis [1358]
Expression system: Not provided
Length: 14 nucleotides
Theoretical weight: 4.05 KDa
DNA (5'-D(*CP*TP*CP*TP*TP*TP*(3DR)P*TP*TP*TP*CP*TP*CP*G)-3') in PDB entry 1pjj, assembly 1, front view.
DNA (5'-D(*CP*TP*CP*TP*TP*TP*(3DR)P*TP*TP*TP*CP*TP*CP*G)-3') in PDB entry 1pjj, assembly 1, side view.
DNA (5'-D(*CP*TP*CP*TP*TP*TP*(3DR)P*TP*TP*TP*CP*TP*CP*G)-3') in PDB entry 1pjj, assembly 1, top view.
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DNA chain E
Name: DNA (5'-D(*GP*CP*GP*AP*GP*AP*AP*AP*CP*AP*AP*AP*GP*A)-3')
Representative chains: E
Source organism: Lactococcus lactis [1358]
Expression system: Not provided
Length: 14 nucleotides
Theoretical weight: 4.36 KDa
DNA (5'-D(*GP*CP*GP*AP*GP*AP*AP*AP*CP*AP*AP*AP*GP*A)-3') in PDB entry 1pjj, assembly 1, front view.
DNA (5'-D(*GP*CP*GP*AP*GP*AP*AP*AP*CP*AP*AP*AP*GP*A)-3') in PDB entry 1pjj, assembly 1, side view.
DNA (5'-D(*GP*CP*GP*AP*GP*AP*AP*AP*CP*AP*AP*AP*GP*A)-3') in PDB entry 1pjj, assembly 1, top view.
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