PDBe 1pja

X-ray diffraction
2.7Å resolution

The crystal structure of palmitoyl protein thioesterase-2 reveals the basis for divergent substrate specificities of the two lysosomal thioesterases (PPT1 and PPT2)

Released:

Function and Biology Details

Reaction catalysed:
Palmitoyl-[protein] + H(2)O = palmitate + [protein]. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysosomal thioesterase PPT2 Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.34 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UMR5 (Residues: 1-302; Coverage: 100%)
Gene name: PPT2
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P6222
Unit cell:
a: 148.52Å b: 148.52Å c: 152.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.251 0.221 0.242
Expression system: Spodoptera frugiperda