› 1pgp

X-ray diffraction
2.5Å resolution

CRYSTALLOGRAPHIC STUDY OF COENZYME, COENZYME ANALOGUE AND SUBSTRATE BINDING IN 6-PHOSPHOGLUCONATE DEHYDROGENASE: IMPLICATIONS FOR NADP SPECIFICITY AND THE ENZYME MECHANISM

Released:
DOI: 10.2210/pdb1pgp/pdb
PDB entry 1pgp coloured by chain, front view.
PDB entry 1pgp coloured by chain, side view.
PDB entry 1pgp coloured by chain, top view.
Source organism: Ovis aries
Primary publication:
Crystallographic study of coenzyme, coenzyme analogue and substrate binding in 6-phosphogluconate dehydrogenase: implications for NADP specificity and the enzyme mechanism.
Adams MJ, Ellis GH, Gover S, Naylor CE, Phillips C
Structure 2 651-68 (1994)
PMID: 7922042

Function and Biology Details

Reaction catalysed: 
6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO(2) + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-phosphogluconate dehydrogenase, decarboxylating Chain: A
Molecule details ›
Chain: A
Length: 482 amino acids
Theoretical weight: 52.91 KDa
Source organism: Ovis aries
Expression system: Not provided
UniProt:
  • Canonical: P00349 (Residues: 2-483; Coverage: 100%)
Gene name: PGD
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 6PG 1 x 6PG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 72.74Å b: 148.4Å c: 102.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.17 not available
Expression system: Not provided

Quick links

Citations

3 review citations

Evolution of protein fold in the presence of functional constraints.
Andreeva et al. (2006)
2 more

8 mentions without citation

6-Phosphogluconate dehydrogenase and its crystal structures.
Hanau et al. (2022)
7 more