1pdi Citations

Three-dimensional structure of bacteriophage T4 baseplate.

Abstract

The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three-dimensional structure of the baseplate-tail tube complex determined to a resolution of 12 A by cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the center of the dome is a needle-like structure that was previously identified as a cell puncturing device. We have identified the locations of six proteins with known atomic structures, and established the position and shape of several other baseplate proteins. The baseplate structure suggests a mechanism of baseplate triggering and structural transition during the initial stages of T4 infection.



Related citations provided by authors (2)

  1. Crystal Structure of a Heat-and Protease-Stable Part of the Bacteriophage T4 Short Tail Fibre. van Raaij MJ, Schoehn G, Burda MR, Miller S J. Mol. Biol. 314 1137-1146 (2001)
  2. Structure of the receptor-binding domain of the bacteriophage T4 short tail fibre. Thomassen E, Gielen G, Schuetz M, Miller S, van Raaij MJ To be published -