› 1p8p

X-ray diffraction
2.5Å resolution

Structural and Functional Importance of First-Shell Metal Ligands in the Binuclear Manganese Cluster of Arginase I.

Released:
DOI: 10.2210/pdb1p8p/pdb
PDB entry 1p8p coloured by chain, front view.
PDB entry 1p8p coloured by chain, side view.
PDB entry 1p8p coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural and functional importance of first-shell metal ligands in the binuclear manganese cluster of arginase I.
Cama E, Emig FA, Ash DE, Christianson DW
Biochemistry 42 7748-58 (2003)
PMID: 12820884

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-ornithine + urea
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139779 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginase-1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 314 amino acids
Theoretical weight: 34.01 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07824 (Residues: 6-319; Coverage: 97%)
Gene name: Arg1
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

1 bound ligand:
Ligand MN 6 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P32
Unit cell:
a: 89Å b: 89Å c: 115.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.237 0.263
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Arginase-1 deficiency.
Sin et al. (2015)
18 other articles

1 mention without citation

Formiminoglutamase from Trypanosoma cruzi is an arginase-like manganese metalloenzyme.
Hai et al. (2013)