PDBe 1p5e

X-ray diffraction
2.22Å resolution

The structure of phospho-CDK2/cyclin A in complex with the inhibitor 4,5,6,7-tetrabromobenzotriazole (TBS)

Released:
Source organism: Homo sapiens
Primary publication:
Alternative binding modes of an inhibitor to two different kinases.
Eur. J. Biochem. 270 3174-81 (2003)
PMID: 12869192

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-A2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 258 amino acids
Theoretical weight: 29.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20248 (Residues: 175-432; Coverage: 60%)
Gene names: CCN1, CCNA, CCNA2
Sequence domains:
Structure domains: Cyclin A; domain 1
Cyclin-dependent kinase 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 299 amino acids
Theoretical weight: 34.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P24941 (Residues: 1-298; Coverage: 100%)
Gene names: CDK2, CDKN2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 73.536Å b: 133.947Å c: 148.424Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.219 0.257
Expression system: Escherichia coli