1p52 Citations

The putative catalytic bases have, at most, an accessory role in the mechanism of arginine kinase.

J. Biol. Chem. 278 26952-7 (2003)
Cited: 29 times
EuropePMC logo PMID: 12732621

Abstract

Arginine kinase is a member of the phosphagen kinase family that includes creatine kinase and likely shares a common reaction mechanism in catalyzing the buffering of cellular ATP energy levels. Abstraction of a proton from the substrate guanidinium by a catalytic base has long been thought to be an early mechanistic step. The structure of arginine kinase as a transition state analog complex (Zhou, G., Somasundaram, T., Blanc, E., Parthasarathy, G., Ellington, W. R., and Chapman, M. S. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 8449-8454) showed that Glu-225 and Glu-314 were the only potential catalytic residues contacting the phosphorylated nitrogen. In the present study, these residues were changed to Asp, Gln, and Val or Ala in several single and multisite mutant enzymes. These mutations had little impact on the substrate binding constants. The effect upon activity varied with reductions in kcat between 3000-fold and less than 2-fold. The retention of significant activity in some mutants contrasts with published studies of homologues and suggests that acid-base catalysis by these residues may enhance the rate but is not absolutely essential. Crystal structures of mutant enzymes E314D at 1.9 A and E225Q at 2.8 A resolution showed that the precise alignment of substrates is subtly distorted. Thus, pre-ordering of substrates might be just as important as acid-base chemistry, electrostatics, or other potential effects in the modest impact of these residues upon catalysis.

Articles - 1p52 mentioned but not cited (3)

  1. A tyrosine kinase and its activator control the activity of the CtsR heat shock repressor in B. subtilis. Kirstein J, Zühlke D, Gerth U, Turgay K, Hecker M. EMBO J. 24 3435-3445 (2005)
  2. Crystal structures of TM0549 and NE1324--two orthologs of E. coli AHAS isozyme III small regulatory subunit. Petkowski JJ, Chruszcz M, Zimmerman MD, Zheng H, Skarina T, Onopriyenko O, Cymborowski MT, Koclega KD, Savchenko A, Edwards A, Minor W. Protein Sci. 16 1360-1367 (2007)
  3. The Receptor Site and Mechanism of Action of Sodium Channel Blocker Insecticides. Zhang Y, Du Y, Jiang D, Behnke C, Nomura Y, Zhorov BS, Dong K. J. Biol. Chem. 291 20113-20124 (2016)


Reviews citing this publication (2)

  1. Chemical biology of protein arginine modifications in epigenetic regulation. Fuhrmann J, Clancy KW, Thompson PR. Chem Rev 115 5413-5461 (2015)
  2. Relating structure to mechanism in creatine kinase. McLeish MJ, Kenyon GL. Crit Rev Biochem Mol Biol 40 1-20 (2005)

Articles citing this publication (24)

  1. Evolution of the arginine kinase gene family. Uda K, Fujimoto N, Akiyama Y, Mizuta K, Tanaka K, Ellington WR, Suzuki T. Comp Biochem Physiol Part D Genomics Proteomics 1 209-218 (2006)
  2. Structural studies of human brain-type creatine kinase complexed with the ADP-Mg2+-NO3- -creatine transition-state analogue complex. Bong SM, Moon JH, Nam KH, Lee KS, Chi YM, Hwang KY. FEBS Lett 582 3959-3965 (2008)
  3. Arginine kinase: joint crystallographic and NMR RDC analyses link substrate-associated motions to intrinsic flexibility. Niu X, Bruschweiler-Li L, Davulcu O, Skalicky JJ, Brüschweiler R, Chapman MS. J Mol Biol 405 479-496 (2011)
  4. Intrinsic domain and loop dynamics commensurate with catalytic turnover in an induced-fit enzyme. Davulcu O, Flynn PF, Chapman MS, Skalicky JJ. Structure 17 1356-1367 (2009)
  5. The crystal structure of Trypanosoma cruzi arginine kinase. Fernandez P, Haouz A, Pereira CA, Aguilar C, Alzari PM. Proteins 69 209-212 (2007)
  6. Metabolic Depression is Delayed and Mitochondrial Impairment Averted during Prolonged Anoxia in the ghost shrimp, Lepidophthalmus louisianensis (Schmitt, 1935). Holman JD, Hand SC. J Exp Mar Bio Ecol 376 85-93 (2009)
  7. Rate-limiting domain and loop motions in arginine kinase. Davulcu O, Skalicky JJ, Chapman MS. Biochemistry 50 4011-4018 (2011)
  8. Arginine kinase: differentiation of gene expression and protein activity in the red imported fire ant, Solenopsis invicta. Wang H, Zhang L, Zhang L, Lin Q, Liu N. Gene 430 38-43 (2009)
  9. The structure of lombricine kinase: implications for phosphagen kinase conformational changes. Bush DJ, Kirillova O, Clark SA, Davulcu O, Fabiola F, Xie Q, Somasundaram T, Ellington WR, Chapman MS. J Biol Chem 286 9338-9350 (2011)
  10. Crystal structure of shrimp arginine kinase in binary complex with arginine-a molecular view of the phosphagen precursor binding to the enzyme. López-Zavala AA, García-Orozco KD, Carrasco-Miranda JS, Sugich-Miranda R, Velázquez-Contreras EF, Criscitiello MF, Brieba LG, Rudiño-Piñera E, Sotelo-Mundo RR. J Bioenerg Biomembr 45 511-518 (2013)
  11. Exploring the role of the active site cysteine in human muscle creatine kinase. Wang PF, Flynn AJ, Naor MM, Jensen JH, Cui G, Merz KM, Kenyon GL, McLeish MJ. Biochemistry 45 11464-11472 (2006)
  12. Phosphagen kinase in Schistosoma japonicum: characterization of its enzymatic properties and determination of its gene structure. Tokuhiro S, Uda K, Yano H, Nagataki M, Jarilla BR, Suzuki T, Agatsuma T. Mol Biochem Parasitol 188 91-98 (2013)
  13. Crystal structures of arginine kinase in complex with ADP, nitrate, and various phosphagen analogs. Clark SA, Davulcu O, Chapman MS. Biochem Biophys Res Commun 427 212-217 (2012)
  14. Crystallization and X-ray analysis of the Schistosoma mansoni guanidino kinase. Awama AM, Paracuellos P, Laurent S, Dissous C, Marcillat O, Gouet P. Acta Crystallogr Sect F Struct Biol Cryst Commun 64 854-857 (2008)
  15. Molecular cloning and characterization of taurocyamine kinase from Clonorchis sinensis: a candidate chemotherapeutic target. Xiao JY, Lee JY, Tokuhiro S, Nagataki M, Jarilla BR, Nomura H, Kim TI, Hong SJ, Agatsuma T. PLoS Negl Trop Dis 7 e2548 (2013)
  16. The roles of C-terminal loop residues of dimeric arginine kinase from sea cucumber Stichopus japonicus in catalysis, specificity and structure. Zhang JW, Zhao TJ, Wang SL, Guo Q, Liu TT, Zhao F, Wang XC. Int J Biol Macromol 38 203-210 (2006)
  17. Despite its high similarity with monomeric arginine kinase, muscle creatine kinase is only enzymatically active as a dimer. Awama AM, Mazon H, Vial C, Marcillat O. Arch Biochem Biophys 458 158-166 (2007)
  18. The Michaelis Complex of Arginine Kinase Samples the Transition State at a Frequency That Matches the Catalytic Rate. Peng Y, Hansen AL, Bruschweiler-Li L, Davulcu O, Skalicky JJ, Chapman MS, Brüschweiler R. J. Am. Chem. Soc. 139 4846-4853 (2017)
  19. Backbone resonance assignments of the 42 kDa enzyme arginine kinase in the transition state analogue form. Davulcu O, Niu X, Brüschweiler-Li L, Brüschweiler R, Skalicky JJ, Chapman MS. Biomol NMR Assign 8 335-338 (2014)
  20. Phosphagen kinase in Schistosoma japonicum: II. Determination of amino acid residues essential for substrate catalysis using site-directed mutagenesis. Tokuhiro S, Nagataki M, Jarilla BR, Uda K, Suzuki T, Sugiura T, Agatsuma T. Mol Biochem Parasitol 194 56-63 (2014)
  21. Biochemical and structural characterization of a novel arginine kinase from the spider Polybetes pythagoricus. Laino A, Lopez-Zavala AA, Garcia-Orozco KD, Carrasco-Miranda JS, Santana M, Stojanoff V, Sotelo-Mundo RR, Garcia CF. PeerJ 5 e3787 (2017)
  22. Common hydrogen bond interactions in diverse phosphoryl transfer active sites. Summerton JC, Martin GM, Evanseck JD, Chapman MS. PLoS One 9 e108310 (2014)
  23. Sorted gene genealogies and species-specific nonsynonymous substitutions point to putative postmating prezygotic isolation genes in Allonemobius crickets. Noh S, Marshall JL. PeerJ 4 e1678 (2016)
  24. The substrate-free and -bound crystal structures of the duplicated taurocyamine kinase from the human parasite Schistosoma mansoni. Merceron R, Awama AM, Montserret R, Marcillat O, Gouet P. J Biol Chem 290 12951-12963 (2015)